Practical Manual on Advance Techniques in Biochemistry
Electrophoresis is a technique used to separate macromolecules
in a fluid or gel based on their charge, binding affinity, and size under
an electric field. In the year 1807, Ferdinand Frederic Reuss was the first
person to observe electrophoresis. He was from Moscow State
University. Anaphoresis is the electrophoresis of negative charge
particles or anions whereas cataphoresis is electrophoresis of positive
charge ions or cations. Electrophoresis has a wide application in
separating and analysing biomolecules such as proteins, plasmids,
RNA, DNA, nucleic acids.
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